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- Title
Molecular characterization and expression of type-I interferon gene in Labeo rohita.
- Authors
Parhi, Janmejay; Mukherjee, S.; Saxena, Gopalkrishna; Sahoo, Lopamudra; Makesh, M.
- Abstract
Genes coding for type-I interferon (I-IFN) has been cloned from Labeo rohita, a commercially important and widely cultured fish in India and South East Asia. In the present study, full-length gene of I-IFN was amplified and sequenced. The sequence analysis revealed that I-IFN consists of 1,786 bp genomic sequence with four introns and five exons and an ORF of 546 bp encoding for a putative protein of 181 amino acids. The mature protein has a molecular weight of 18.97 kDa and consists of 158 amino acids and a signal peptide of 23 amino acids at the N terminus. The sequence carries I-IFN signature motif, one glycosylation site, two conserved cystine amino acids and other conserved amino acids. The sequence showed highest similarity to that of Cyprinus carpio (84 %). In silico analysis of the rohu I-IFN protein was done using various bioinformatic tools. The constitutive expression of I-IFN gene was found to be more in spleen compared to gill and kidney in real time PCR assay. Expression of I-IFN increased about 20-fold in cultured kidney cell 2 h after induction with poly I:C and showed maximum expression at 8 h post-induction.
- Subjects
ROHU; INTERFERON genetics; GENE expression; BIOINFORMATICS; AMINO acid sequence; CARP
- Publication
Molecular Biology Reports, 2014, Vol 41, Issue 5, p2979
- ISSN
0301-4851
- Publication type
Article
- DOI
10.1007/s11033-014-3155-0