We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Urotensin II promotes the production of LTC in rat aortic adventitial fibroblasts through NF-κB-5-LO pathway by p38 MAPK and ERK activations.
- Authors
Dong, Xiao; Ye, Xiaojin; Song, Nana; Zhao, Jing; Di, Beibing; Peng, Fen; Tang, Chaoshu; Ding, Wenhui
- Abstract
Adventitia is the outer part of the arterial wall where the inflammatory response often occurs. Urotensin II (UII) is a potent vasoconstrictive peptide that also promotes the inflammatory process in patients with cardiovascular disease. Leukotriene C (LTC), a lipid mediator, was recently found to play a role in the inflammatory process in the artery. We hypothesized that the adventitia is one of the resources of LTC and that UII may promote LTC production through the 5-LO (5-lipoxygenase) pathway in adventitial fibroblasts. Rat adventitial fibroblasts were isolated and incubated in serum-free medium with either UII alone or in combination with inhibitors of p38 MAPK, ERK, and UII receptors. The expression of 5-LO was detected using real-time polymerase chain reaction and Western blot. The translocation and binding activity of nuclear factor (NF)-κB were measured using immunofluorescence and electrophoretic mobility shift assay, respectively. The production of LTC was measured by enzyme-linked immunosorbent assay. The results indicated that: (1) adventitial fibroblasts were a source of LTC production; (2) UII increased the expression of the 5-LO mRNA and the protein by NF-κB activation through p38 MAPK and ERK pathways; and (3) UII promoted the LTC release in fibroblasts through the 5-LO pathway by p38 MAPK and ERK activations. The 5-LO pathway mediates LTC production, which may be a new mechanism in the pathogenesis of the vascular adventitial inflammation caused by UII.
- Subjects
UROTENSINS; FIBROBLASTS; NF-kappa B; MITOGEN-activated protein kinase phosphatases; ENZYME activation; LABORATORY rats; GENE expression
- Publication
Heart & Vessels, 2013, Vol 28, Issue 4, p514
- ISSN
0910-8327
- Publication type
Article
- DOI
10.1007/s00380-012-0291-0