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- Title
LC-MS/MS Evaluation of Pyrrolizidine Alkaloids Profile in Relation to Safety of Comfrey Roots and Leaves from Polish Sources.
- Authors
Kimel, Katarzyna; Godlewska, Sylwia; Gleńsk, Michał; Gobis, Katarzyna; Ośko, Justyna; Grembecka, Małgorzata; Krauze-Baranowska, Mirosława
- Abstract
Comfrey (Symphytum officinale L.) has a long tradition of use in the treatment of musculoskeletal disorders. However, due to hepatotoxic pyrrolizidine alkaloids (PAs), the EMA restricts the use of comfrey root (CR) to external use only and for short periods of time. Recent studies indicate a low permeability of PAs across the skin, calling into question the safety of topical application of products containing comfrey preparations. The aim of our work was to develop and validate an HPLC method enabling the separation of isomeric PAs from comfrey and, on this basis, to assess the potential toxicity of CR and comfrey leaf (CL) obtained from various Polish sources. The qualitative and quantitative analysis of PAs via HPLC-MS/MS was performed in MRM mode. The results obtained confirmed a lower content of PAs in CL than in CR and showed a wide variation in the composition of PAs in CR, with a much more stable profile of PAs in CL. Factor analysis confirmed that CRs and CLs differ in PA content, which is influenced by the growth conditions and geographical origin. The determined concentrations of PAs prove that in some CRs available on the Polish herbal market, the content of PAs may exceed the daily dose considered safe.
- Subjects
PYRROLIZIDINES; TOPICAL drug administration; LIQUID chromatography-mass spectrometry; FACTOR analysis; MUSCULOSKELETAL system diseases
- Publication
Molecules, 2023, Vol 28, Issue 16, p6171
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules28166171