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- Title
Biosynthesis of rubradirin as an ansamycin antibiotic from Streptomyces achromogenes var. rubradiris NRRL3061.
- Authors
Chun-Gyu Kim; Lamichhane, Janardan; Kwang-Il Song; Nguyen, Van D.; Dong-Hee Kim; Tae-Sung Jeong; Sung-Ho Kang; Kyoung-Wook Kim; Maharjan, Jyoti; Young-Soo Hong; Jae Seon Kang; Jin-Cheol Yoo; Jung-Joon Lee; Tae-Jin Oh; Kwangkyoung Liou; Jae Kyung Sohng
- Abstract
The four overlapping cosmids from the rubradirin producer , Streptomyces achromogenes var rubradiris NRRL 3061, have 58 ORFs within a 105.6 kb fragment. These ORFs harbored essential genes responsible for the formation and attachment of four distinct moieties, along with the genes associated with regulatory, resistance, and transport functions. The PKS ( rubA) and glycosyltransferase ( rubG2) genes were disrupted in order to demonstrate a complete elimination of rubradirin production. The rubradirin biosynthetic pathway was proposed based on the putative functions of the gene products, the functional identification of sugar genes, and the mutant strains.
- Subjects
BIOCHEMISTRY; BIOSYNTHESIS; ORGANIC synthesis; ANTIBIOTICS; STREPTOMYCES; GENETICS; HEREDITY; MOIETIES (Chemistry); GLYCOSYLTRANSFERASE genes
- Publication
Archives of Microbiology, 2008, Vol 189, Issue 5, p463
- ISSN
0302-8933
- Publication type
Article
- DOI
10.1007/s00203-007-0337-3