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- Title
Identification of the fertility restoration locus, Rfo, in radish, as a member of the pentatricopeptide-repeat protein family.
- Authors
Desloire, Sophie; Gherbi, Hassen; Laboui, Wassila; Marhadour, Sylvie; Clouet, Vanessa; Cattolico, Laurence; Falentin, Cyril; Giancola, Sandra; Renard, Michel; Budar, Françoise; Small, Ian; Caboche, Michel; Delourme, Régine; Bendahmane, Abdelhafid
- Abstract
Ogura cytoplasmic male sterility (CMS) in radish (Raphanus sativus) is caused by an aberrant mitochondrial gene, Orf 138, that prevents the production of functional pollen without affecting female fertility. Rfo, a nuclear gene that restores male fertility, alters the expression of Orf 138 at the post-transcriptional level. The Ogura CMS Rfo two-component system is a useful model for investigating nuclear-cytoplasmic interactions, as well as the physiological basis of fertility restoration. Using a combination of positional cloning and microsynteny analysis of Arabidopsis thaliana and radish, we genetically and physically delimited the Rfo locus to a 1-kb DNA segment. Analysis of this segment shows that Rfo is a member of the pentatricopeptide repeat (PPR) family. In Arabidopsis, this family contains more than 450 members of unknown function, although most of them are predicted to be targeted to mitochondria and chloroplasts and are thought to have rolesin organellar gene expression.
- Subjects
RADISHES; MALE infertility; RESTORATION ecology; MITOCHONDRIA; PEPTIDES; PROTEINS; ARABIDOPSIS; GENE expression
- Publication
EMBO Reports, 2003, Vol 4, Issue 6, p588
- ISSN
1469-221X
- Publication type
Article
- DOI
10.1038/sj.embor.embor848