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- Title
Cross-competition for TATA-binding Protein between TATA Boxes of the Selenocysteine tRNA<sup>[Ser]Sec</sup> Promoter and RNA Polymerase II Promoters.
- Authors
Jin Mo Park; Hatfield, Dolph L.; Byeong Jae Lee
- Abstract
In this study we show that the TATA-binding protein (TBP) interacts with the selenocysteine tRNA[Ser]Sec TATA element in a fashion analogous to the TBP-TATA interaction in RNA polymerase (Pol) II-transcribed genes even though the gene is transcribed by Pol III. Recombinant TBPs expressed in Escherichia coli bound to the tRNA|SerlSec TATA element. A factor was detected in Xenopus oocyte extracts which contain TBP and bind to the TATA boxes of the tRNA[Ser]Sec gene and various class II genes. Transcription of the microinjected tRNA|Scr|Sec gene was inhibited in Xenopus oocytes by coinjection with the TATA box of the adenovirus major late promoter (AdMLP). Transcription of a 5S gene was not affected under these conditions. These results suggest that the tRNA[Ser]sec gene recruits TBP in a manner similar to that of TATA-dependent Pol II-transcribed genes and differently from that of Pol Ill-transcribed genes lacking a TATA box.
- Subjects
CARRIER proteins; TRANSFER RNA; SELENOCYSTEINE; RNA polymerase II; ESCHERICHIA coli
- Publication
Molecules & Cells (Springer Nature), 1997, Vol 7, Issue 1, p72
- ISSN
1016-8478
- Publication type
Article
- DOI
10.1016/s1016-8478(23)13264-x