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- Title
The production of the eosinophil proteins ECP and EPX/ EDN are regulated in a reciprocal manner.
- Authors
Jönsson, Ulla‐Britt; Blom, Kristin; Stålenheim, Gunnemar; Håkansson, Lena Douhan; Venge, Per
- Abstract
Previous studies showed that the biological activity and the eosinophil content of eosinophil cationic protein ( ECP, RNase 3) are determined by single-nucleotide polymorphisms ( SNPs) in the ECP ( RNase3) gene . In this study, we report the prevalence of a common SNP in the eosinophil protein x/eosinophil-derived neurotoxin ( EPX/ EDN, RNase2) and the association with the cellular contents of EPX/ EDN and ECP. The genes were sequenced and the EPX/ EDN405(G>C) rs2013109 SNPs were also determined by TaqMan 5′nuclease allelic discrimination assay. ECP and EPX/ EDN in purified eosinophils or in whole blood extracts were analysed by sensitive immunoassays. The study included 379 non-allergic and allergic subjects. The genotype prevalence of the EPX/ EDN405(G>C) polymorphism was GG 59%, GC 36% and CC 6%. The cellular contents of ECP and EPX/ EDN were related in a reciprocal fashion with the sums of the protein contents being constant. The contents were associated with the ECP562(G>C) rs2233860 and EPX/ EDN405(G>C) gene polymorphisms. The cellular content of eosinophil peroxidase ( EPO) was not associated with the ECP and EPX/ EDN genotypes. The prevalence of the EPX/ EDN405(G>C) genotypes and the contents of the proteins were similar in non-allergic and allergic subjects. The production and storage of the two ancestral proteins, ECP and EPX/ EDN likely share common regulatory mechanisms, which result in opposing productions of the two proteins.
- Subjects
EOSINOPHILS; BASIC proteins; SINGLE nucleotide polymorphisms; GENETIC polymorphisms; RIBONUCLEASE III; BLOOD sampling
- Publication
APMIS, 2014, Vol 122, Issue 4, p283
- ISSN
0903-4641
- Publication type
Article
- DOI
10.1111/apm.12142