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- Title
Analysis of Molecular Mechanisms Underlying the Transformation of Compost Organic Waste Using a Combination of Electrophoresis, Size-Exclusion Chromatography, and Absorption Spectroscopy.
- Authors
Trubetskoy, O. A.; Trubetskaya, O. E.
- Abstract
The humin-like substances of compost at the beginning of transformation and after 130 days were studied using a set of analytical methods, including analytical electrophoresis in 10% polyacrylamide gel in the presence of denaturing agents, absorption spectroscopy, and size-exclusion chromatography on Sephadex G-75. It is shown that the transformation of organic waste during composting is accompanied by a decrease in the share of highest molecular size fraction and the formation of a significant amount of the fractions with the lowest molecular size with a concurrent increase in the share of aromatic components in humic-like substances. The used toolkit of analytical methods is applicable to assess the efficiency of various composting processes for organic household and industrial waste. This approach can be also useful for soil remediation.
- Subjects
ORGANIC wastes; POLYACRYLAMIDE gel electrophoresis; INDUSTRIAL wastes; COMPOSTING; MOLECULAR size; DENATURING gradient gel electrophoresis
- Publication
Eurasian Soil Science, 2022, Vol 55, Issue 8, p1028
- ISSN
1064-2293
- Publication type
Article
- DOI
10.1134/S1064229322080142