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- Title
Distinct hemogenic potential of endothelial cells and CD41<sup>+</sup> cells in mouse embryos.
- Authors
Hashimoto, Kazuaki; Fujimoto, Tetsuhiro; Shimoda, Yuri; Xin Huang; Sakamoto, Hiroshi; Ogawa, Minetaro
- Abstract
Definitive hematopoietic progenitor cells have been thought to develop from the vascular endothelium located in the aorta-gonad-mesonephros region of the mouse embryo. However, several recent findings have suggested that most hematopoietic progenitors are derived from non-endothelial precursor cells expressing CD41. We characterized two distinct precursor populations of definitive hematopoietic cell lineages, vascular endothelial (VE)-cadherin+ CD41− CD45− endothelial cells and CD41+ CD45− non-endothelial progenitors, both of which are derived from lateral mesoderm. VE-cadherin+ endothelial cells obtained from cultures of differentiating embryonic stem cells possessed hematopoietic potential encompassing erythroid, myeloid and B lymphoid lineages, whereas CD41+ progenitors lacked the B lymphopoietic potential. VE-cadherin+ endothelial cells in the lower trunk of the embryo proper showed a significant potential for initiating B lymphopoiesis in cultures, while endothelial cells in the yolk sac appeared to have a bias for myeloerythropoietic differentiation. CD41+ progenitors isolated from yolk sac and embryo proper were capable of generating multiple hematopoietic lineages, although mast cell precursors were exclusively enriched in CD41+ progenitors in the yolk sac. These results suggest that hemogenic endothelial cells and CD41+ progenitors possess distinct hematopoietic potential depending on the tissues in which they reside.
- Subjects
EMBRYONIC stem cells; ENDOTHELIUM; VASCULAR endothelium; BLOOD vessels; HUMAN cloning; STEM cells
- Publication
Development, Growth & Differentiation, 2007, Vol 49, Issue 4, p287
- ISSN
0012-1592
- Publication type
Article
- DOI
10.1111/j.1440-169X.2007.00925.x