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- Title
Frequencies of interleukin-5 mRNA-producing cells in healthy individuals and in immunoglobulin-deficient patients, measured by in situ hybridization.
- Authors
Smith, C. I. E.; Möller, G.; Severinson, E.; Hammarström, L.
- Abstract
Interleukin-5 (IL-5) has previously been demonstrated to enhance immunoglobulin synthesis, especially IgA, Thus, it could be hypothesized that a defect production of IL-5 may cause immunoglobulin deficiency. We have analysed the frequency of IL-5 mRNA-producing cells in healthy adults and in patients with common variable immunodeficiency or selective IgA deficiency. Unstimulated lymphocytes were rarely found to synthesize IL-5 as measured by in situ hybridization. However, pokeweed mitogen and several other activating ligands induced the synthesis of IL-5 mRNA in peripheral blood and spleen lymphocyte cultures. After pokeweed mitogen activation, the number of IL-5 mRNA-producing cells most often peaked on day 3 with a maximal frequency of around 1 -2% of mononuclear cells. In a kinetic study we were unable to detect any peak frequency differences between healthy controls (mean 0-44%) and 20 patients (mean 0.58%). Thus, although IL- 5 has been reported to be an important regulator of IgA synthesis, a defect production does not seem to be the underlying mechanism in human immunoglobulin deficiency.
- Subjects
INTERLEUKIN-5; MESSENGER RNA; IMMUNOGLOBULINS; IN situ hybridization; LEUCOCYTES; MITOGENS
- Publication
Clinical & Experimental Immunology, 1990, Vol 81, Issue 3, p417
- ISSN
0009-9104
- Publication type
Article