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- Title
The catE gene of Bacillus licheniformis M2-7 is essential for growth in benzopyrene, and its expression is regulated by the Csr system.
- Authors
Morales-Blancas, Giselle Yamilet; Reyna-Terán, José Daniel; Hernández-Eligio, José Alberto; Ortuño-Pineda, Carlos; Toribio-Jiménez, Jeiry; Rodríguez-Barrera, Miguel Ángel; Toledo-Hernández, Erubiel; Rojas-Aparicio, Augusto; Romero-Ramírez, Yanet
- Abstract
Benzopyrene is a high-molecular-weight polycyclic aromatic hydrocarbon that is highly recalcitrant and induces carcinogenic effects. CsrA is a conserved regulatory protein that controls the translation and stability of its target transcripts, having negative or positive effects depending on the target mRNAs. It is known that Bacillus licheniformis M2-7 has the ability to grow and survive in certain concentrations of hydrocarbons such as benzopyrene, prompted in part by CsrA, as is present in gasoline. However, there are a few studies that reveal the genes involved in that process. To identify the genes involved in the Bacillus licheniformis M2-7 degradation pathway, the plasmid pCAT-sp containing a mutation in the catE gene was constructed and used to transform B. licheniformis M2-7 and generate a CAT1 strain. We determined the capacity of the mutant B. licheniformis (CAT1) to grow in the presence of glucose or benzopyrene as a carbon source. We observed that the CAT1 strain presented increased growth in the presence of glucose but a statistically considerable decrease in the presence of benzopyrene compared with the wild-type parental strain. Additionally, we demonstrated that the Csr system positively regulates its expression since it was observed that the expression of the gene in the mutant strain LYA12 (M2-7 csrA:: Sp, SpR) was considerably lower than that in the wild-type strain. We were thus able to propose a putative regulation model for catE gene in B. licheniformis M2-7 strain by CsrA regulator in the presence of benzopyrene.
- Subjects
BACILLUS licheniformis; GENE expression; PLASMIDS; BENZOPYRENE; POLYCYCLIC aromatic hydrocarbons; SOCIAL responsibility of business
- Publication
World Journal of Microbiology & Biotechnology, 2023, Vol 39, Issue 7, p1
- ISSN
0959-3993
- Publication type
Article
- DOI
10.1007/s11274-023-03630-3