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- Title
Astragaloside IV protects against autoimmune myasthenia gravis in rats via regulation of mitophagy and apoptosis.
- Authors
Zhang, Jingjing; Huang, Jiayan; Lan, Jinlian; Li, Qing; Ke, Lingling; Jiang, Qilong; Li, Yanwu; Zhang, Han; Zhong, Huiya; Yang, Peidan; Chen, Tongkai; Song, Yafang
- Abstract
Astragaloside IV (AS-IV) has various pharmacological effects, including antioxidant and immunoregulatory properties, which can improve myasthenia gravis (MG) symptoms. However, the potential mechanism underlying the effects of AS-IV on MG remains to be elucidated. The present study aimed to investigate whether AS-IV has a therapeutic effect on MG and its potential mechanism of action. By subcutaneously immunizing rats with R97-116 peptide, an experimental autoimmune (EA) MG rat model was established. AS-IV (40 or 80 mg/kg/day) treatment was then applied for 28 days after modeling. The results demonstrated that AS-IV significantly ameliorated the weight loss, Lennon score and pathological changes in the gastrocnemius muscle of EAMG rats compared with the model group. Additionally, the levels of acetylcholine receptor antibody (AChR-Ab) were significantly decreased, whereas mitochondrial function [ATPase and cytochrome c (Cyt-C) oxidase activities] and ultrastructure were improved in the AS-IV treated rats. Moreover, the mRNA and protein expression levels of phosphatase and tensin homolog-induced putative kinase 1, Parkin, LC3II and Bcl-2, key signaling molecules for mitophagy and apoptosis, were upregulated, whereas the mRNA and protein expression levels of p62, Cyt-C, Bax, caspase 3 and caspase 9 were downregulated following AS-IV intervention. In conclusion, AS-IV may protect against EAMG in a rat model by modulating mitophagy and apoptosis. These findings indicated the potential mechanism underlying the effects of AS-IV on MG and provided novel insights into treatment strategies for MG.
- Subjects
MYASTHENIA gravis; LABORATORY rats; APOPTOSIS; RATS; CYTOCHROME oxidase; CHOLINERGIC receptors; MITOGEN-activated protein kinase phosphatases
- Publication
Molecular Medicine Reports, 2024, Vol 30, Issue 1, pN.PAG
- ISSN
1791-2997
- Publication type
Article
- DOI
10.3892/mmr.2024.13253