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- Title
Antibacterial Biopolymer Gel Coating on Meshes Used for Abdominal Hernia Repair Promotes Effective Wound Repair in the Presence of Infection.
- Authors
Benito-Martínez, Selma; Pérez-Köhler, Bárbara; Rodríguez, Marta; García-Moreno, Francisca; Gómez-Gil, Verónica; Pascual, Gemma; Bellón, Juan Manuel
- Abstract
Prosthetic mesh infection is a devastating complication of abdominal hernia repair which impairs natural healing in the implant area, leading to increased rates of patient morbidity, mortality, and prolonged hospitalization. This preclinical study was designed to assess the effects on abdominal wall tissue repair of coating meshes with a chlorhexidine or rifampicin-carboxymethylcellulose biopolymer gel in a Staphylococcus aureus (S. aureus) infection model. Partial abdominal wall defects were created in New Zealand white rabbits (n = 20). Four study groups were established according to whether the meshes were coated or not with each of the antibacterial gels. Three groups were inoculated with S. aureus and finally repaired with lightweight polypropylene mesh. Fourteen days after surgery, implanted meshes were recovered for analysis of the gene and protein expression of collagens, macrophage phenotypes, and mRNA expression of vascular endothelial growth factor (VEGF) and matrix metalloproteinases (MMPs). Compared to uncoated meshes, those coated with either biopolymer gel showed higher collagen 1/3 messenger RNA and collagen I protein expression, relatively increased VEGF mRNA expression, a significantly reduced macrophage response, and lower relative amounts of MMPs mRNAs. Our findings suggest that following mesh implant these coatings may help improving abdominal wall tissue repair in the presence of infection.
- Subjects
NEW Zealand; HERNIA; WOUND healing; VASCULAR endothelial growth factors; ABDOMINAL wall; MATRIX metalloproteinases; BIOPOLYMERS; MESSENGER RNA
- Publication
Polymers (20734360), 2021, Vol 13, Issue 14, p2371
- ISSN
2073-4360
- Publication type
Article
- DOI
10.3390/polym13142371