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- Title
In Vitro Studies on Callus Induction of Moringa Peregrina (Forssk) Fiori and Antifungal Activity of Plant Extracts.
- Authors
Alrayes, Linda Naim; Shatnawi, Mohamad Awad; Wesam Moh'd Hadi Al Khateeb
- Abstract
In this study a satisfactory method for callus induction and growth has been developed for Moringa peregrina. Maximum callus size was observed on MS medium supplemented with 1.0 mg/L 2,4-D, while maximum callus weight was achieved on MS medium supplemented with 2.0 mg/L 2,4-D. Antifungal activity of aqueous, methanolic, ethanolic, and acetonic extracts of both in vitro (plantlets and callus) and ex vitro plant were evaluated by the agar well-diffusion method against several fungal strains including: Aspergillus brasiliensis, Aspergillus italicium, Aspergillus niger brown (mutant), Aspergillus niger black, Penicillium digilatum, Phytophthora infestans and Candida albicans. In vitro aqueous extract was found to be more effective compared to the positive control and the ex vitro aqueous extract against Pencillium digilatum and Phytophthora infestans, while in vitro methanolic extract was more effective than positive control against Candida albicans.
- Subjects
PLANT extracts; ANTIFUNGAL agents; CALLUS; MORINGA; ASPERGILLUS niger; CANDIDA albicans
- Publication
Jordan Journal of Agricultural Sciences, 2018, Vol 14, Issue 2, p147
- ISSN
1815-8625
- Publication type
Article