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- Title
Parentage assignment in hybrid abalones ( Haliotis rufescens × Haliotis discus hannai) based on microsatellite DNA markers.
- Authors
Lafarga‐de la Cruz, Fabiola; Aguilar‐Espinoza, Andrea; Gallardo‐Escárate, Cristian
- Abstract
Parentage analysis in aquaculture determines genealogical relationships between broodstock and progeny when the parents are unknown. Thus, parentage analysis is a useful tool to establish pedigree reports in molecular-assisted selection programs. Here, we evaluated 10 heterologous microsatellite markers for parentage assignment in abalone hybrids produced from 43 abalone broodstocks of red abalone ( Haliotis rufescens) and Japanese abalone ( H. discus hannai). The allele frequencies, exclusion probabilities and broodstock contributions were calculated using CERVUS, PAPA and GERUD software. The polymorphic information content ( PIC) values showed that most of the microsatellite loci were highly informative (>0.7) and more than 90% of parentage assignment was possible with a minimum of 5-6 microsatellite markers. Parentage assignment for hybrid and pure-red progeny showed a better performance than pure-Japanese progeny. This result could be due to the high level of allele loss in the parental genotypes. In addition, results indicated that only two sires contributed over 80% and 90% of red and hybrid progenies, respectively. This study gives a new molecular tool to support marker-assisted selection in abalone hybrids produced in Chile.
- Subjects
MICROSATELLITE repeats; ABALONES; ABALONE genetics; GENETIC markers; RED abalone; PHYSIOLOGY
- Publication
Aquaculture Research, 2015, Vol 46, Issue 1, p216
- ISSN
1355-557X
- Publication type
Article
- DOI
10.1111/are.12169