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- Title
烟草苗移栽后室内与田间根结线虫幼虫侵染动态检测.
- Authors
李宏光; 余萍; 阙劲松; 刘春明; BUDAHN Holger; 李勇军; 朋金娥; 罗红梅; 张绍松
- Abstract
【Objective】 Root-knot nematodes are important pathogens causing serious yield losses in tobacco. The early stage after transplantation is especially sensitive for infection with Meloidogyne species. In order to control effectively the nematode disease, it is necessary to determine the population structure and dynamic as well as to calculate the titer of second stage juveniles(J2) over time. 【Method】 After harvest of the tobacco leaves in autumn 2013 infected plants and their rhizospere soil were sampled. The percentage of the three Meloidogyne species M. arenaria, M. javanica and M. incognita was determined by observing the perineal pattern of the female tail. Soil from the provocation field was used for lab-cultivation of young tobacco seedlings. Infection dynamic was followed from 6 thto 21 stday after transplantation of tobacco seedlings and the results were compared with the infection dynamic of the root-knot nematodes in 2014 in the original tobacco field.【Result】 Three populations of root-knot nematode species were detected in the plant root galls: M. arenaria(52.6 %), M. javanica(31.6%) and M. incognita(15.8 %). J2 in tobacco root tissue was counted from 6 thto 21 stday after transplantation in lab and field. The infected crest-time in lab test was shorter and the infection rate was higher than in the field trial.【Conclusion】 Three different Meloidogyne species were found in a nematode provocation tobacco field. The average of 8.4 J2/50 m L soil was still detected in the soil before transplanting tobacco seedlings. The infection of J2 larvae into tobacco root tissue happened very early in field trial and lab test.
- Publication
Southwest China Journal of Agricultural Sciences, 2018, Vol 31, Issue 6, p1203
- ISSN
1001-4829
- Publication type
Article
- DOI
10.16213/j.cnki.sojas.2018.6.017