We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Two-dimensional pulsed electron spin resonance characterization of <sup>15</sup>N-labeled archaeal Rieske-type ferredoxin
- Authors
Iwasaki, Toshio; Samoilova, Rimma I.; Kounosu, Asako; Dikanov, Sergei A.
- Abstract
Abstract: Two-dimensional electron spin-echo envelope modulation (ESEEM) analysis of the uniformly 15N-labeled archaeal Rieske-type [2Fe–2S] ferredoxin (ARF) from Sulfolobus solfataricus P1 has been conducted in comparison with the previously characterized high-potential protein homologs. Major differences among these proteins were found in the hyperfine sublevel correlation (HYSCORE) lineshapes and intensities of the signals in the (++) quadrant, which are contributed from weakly coupled (non-coordinated) peptide nitrogens near the reduced clusters. They are less pronounced in the HYSCORE spectra of ARF than those of the high-potential protein homologs, and may account for the tuning of Rieske-type clusters in various redox systems.
- Subjects
ELECTRON nuclear double resonance; ELECTRON spin echo envelope modulation spectroscopy; ELECTRON paramagnetic resonance; NUCLEAR magnetic resonance; FOURIER transform optics
- Publication
FEBS Letters, 2009, Vol 583, Issue 21, p3467
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2009.09.050