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- Title
Localization of a putative ClC chloride channel in spinach chloroplasts
- Authors
Teardo, Enrico; Frare, Erica; Segalla, Anna; Marco, Veronica De; Giacometti, Giorgio Mario; Szabò, Ildikò
- Abstract
Abstract: Seven genes seem to encode for putative ClC chloride channels (AtClC-a to AtClC-g) in Arabidopsis thaliana. Their function and localization is still largely unknown. AtClC-f shares considerable sequence similarity with putative ClC channel proteins from Synechocystis, considered to represent the precursor of chloroplasts. We show by biochemical and mass spectrometry analysis that ClC-f is located in the outer envelope membrane of spinach chloroplasts. Consistent with the plastidial localization of ClC-f, p-chlorophenoxy-acetic acid (CPA) reduces photosynthetic activity and the protein is expressed in etioplasts and chloroplasts but not in root tissue. These findings may represent a step toward the molecular identification of ion channel activities in chloroplast membranes.
- Subjects
ION channels; CHLOROPLASTS; PHOTOBIOLOGY; ARABIDOPSIS
- Publication
FEBS Letters, 2005, Vol 579, Issue 22, p4991
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2005.08.005