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- Title
Langerin<sup>+</sup> Dermal DC, but Not Langerhans Cells, Are Required for Effective CD8-Mediated Immune Responses after Skin Scarification with Vaccinia Virus.
- Authors
Seneschal, Julien; Jiang, Xiaodong; Kupper, Thomas S
- Abstract
Skin scarification (s.s.) with vaccinia virus (VACV) is essential for generation of an optimal protective T-cell memory immune response. Dendritic cells (DCs), which are professional antigen-presenting cells, are required for naive T-cell priming and activation. At least three subsets of skin-resident DC have been identified: Langerhans cells (LCs), dermal Langerin+ DC (Lang+dDC), and dermal Langerin- DC (Lang-dDC). Using Langerin-diphtheria toxin receptor mice and established mouse model of VACV delivered by s.s., we demonstrated that Lang+dDC, but not LC, are absolutely required for the induction of a rapid and robust antigen-specific CD8+ T-cell response after s.s. with VACV. The depletion of Lang+dDC led to a significant delay in the priming and proliferation of antigen-specific CD8+ T cells. Moreover, CD8+ T cells generated after VACV s.s. in the absence of Lang+dDC lacked effector cytotoxic functions both in vitro and in vivo. While s.s.-immunized wild-type and LC-depleted mice controlled the progression of OVA257-264 expressing T-cell lymphoma EG7 (injected intradermally), the depletion of Lang+dDC led to rapid lymphoma progression and mortality. These data indicate that of all skin DC subsets, Lang+dDC is the most critical for the generation of robust CD8+ T-cell immunity after s.s. with VACV.
- Subjects
CELL surface antigens; LANGERHANS cells; CD8 antigen; IMMUNE response; SCARIFICATION (Body marking); VACCINIA; DENDRITIC cells
- Publication
Journal of Investigative Dermatology, 2014, Vol 134, Issue 3, p686
- ISSN
0022-202X
- Publication type
Article
- DOI
10.1038/jid.2013.418