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- Title
C/EBPγ Regulates Wound Repair and EGF Receptor Signaling.
- Authors
Melchionna, Roberta; Bellavia, Gabriella; Romani, Marta; Straino, Stefania; Germani, Antonia; Di Carlo, Anna; Capogrossi, Maurizio C; Napolitano, Monica
- Abstract
We aimed at identifying novel regulators of skin wound healing (WH), in an epidermal scratch WH assay, by a small interfering RNA (siRNA) silencing approach. Several transcription factors have been previously reported to affect wound repair. We here show that gene silencing of the transcription factor CAAT enhancer-binding protein γ (C/EBPγ), STAT3, REL, RELA, RELB, SP1, and NFkB impaired WH in vitro, in keratinocytes, whereas E2F and CREBBP silencing accelerated the WH process. We further characterized C/EBPγ, as its silencing yielded the maximal impairment (52.2±12.5%) of scratch wounding (SW). We found that C/EBPγ silencing inhibited both EGF- and serum-induced keratinocyte migration, whereas C/EBPγ overexpression enhanced cell migration to EGF and to serum via the EGFR. Further, C/EBPγ silencing impaired scratch-induced Y1068 and Y1173 EGFR phosphorylation, as well as Y118 paxillin phosphorylation, key molecules regulating cell migration and epidermal WH. Moreover, C/EBPγ levels were induced in keratinocytes, following both SW and EGF stimulation. C/EBPγ siRNA silencing in vivo impaired WH at 3, 5, 7, and 14 days following excisional wounding in mice inhibited both re-epithelialization and granulation tissue formation, and induced a decrease of arteriole number. In conclusion, we here report that C/EBPγ positively regulates wound repair both in vitro and in vivo, at least in part, by affecting EGFR signaling.
- Subjects
CCAAT enhancer binding proteins; EPIDERMAL growth factor receptors; GRANULATION tissue; CELL migration; TRANSCRIPTION factors; PAXILLIN; PHOSPHORYLATION
- Publication
Journal of Investigative Dermatology, 2012, Vol 132, Issue 7, p1908
- ISSN
0022-202X
- Publication type
Article
- DOI
10.1038/jid.2012.51