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- Title
Inhibition of Alloantigen-Primed Memory CD4<sup>+</sup> and CD8<sup>+</sup> T Cells by Hematopoietic Chimerism in Mice.
- Authors
Lan, T.; Chen, J.; Xia, J.; Wang, Y.; Xie, B.; Wang, F.; Qi, Z.
- Abstract
Donor-reactive memory T cells present a special hurdle in transplantation. Although hematopoietic chimerism is effective for inducing donor-specific tolerance, the effects on memory T cells are unclear. Here, we induced stable chimerism and tolerance in mice (Tolerance group, n = 6) by donor-specific transfusion (DST) plus anti-CD154 monoclonal antibody (mAb), avoiding the toxic myeloablative conditioning treatment to assist bone marrow transplantation (DST/aCD154&BMTx). We then transferred memory CD4+ or CD8+ T cells from donor antigen primed mice to the tolerance-induced recipients 4 days after heart transplantation (Tol/CD4+ Tm group and Tol/CD8+ Tm group, n = 6, respectively), but neither of these memory T-cell subsets had an effect on the permanent graft survival (median survival time > 100 days). The unaltered rate of memory T cells in spleen and anergy to donor antigen in vitro demonstrated that these memory T cells were well controlled. The chimerism-promoting protocol DST/aCD154&BMTx produced an immune environment that included high levels of regulatory T cells (Tregs), microchimerism and TGF-β, all of which may act in suppressing the donor-reactive memory CD4+ or CD8+ T cells. These findings have potentially important implications for designing approaches to suppressing memory T cells for success of transplantation.
- Subjects
T cells; BONE marrow transplantation; MONOCLONAL antibodies; IMMUNE system; MOLECULAR cloning
- Publication
Scandinavian Journal of Immunology, 2010, Vol 72, Issue 2, p86
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.2010.02412.x