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- Title
Mycoplasma mycoides ssp. mycoides Biotype Small Colony-Secreted Components Induce Apoptotic Cell Death in Bovine Leucocytes.
- Authors
Dedieu, L.; Chapey, E.; Balcer-Rodrigues, V.
- Abstract
Contagious bovine pleuropneumonia, caused by Mycoplasma mycoides ssp. mycoides biotype small colony ( MmmSC), is one of the most serious cattle diseases in Africa. Several observations suggested that MmmSC had evolved an efficient way to escape the bovine immune responses by triggering host-cell cytotoxicity. This study was implemented to determine whether the cytotoxic effect was due to apoptotic cell death. To that end, bovine blood cells were cultured for up to 3 days in the presence of viable or heat-killed MmmSC compared to unstimulated cultures. The findings provided evidence for a viable MmmSC-induced, time-dependent apoptosis in bovine blood leucocytes, whereas heat-killed MmmSC had no effect. Morphological and physiological changes (evidenced by TUNEL and annexin V staining) typical of apoptosis were observed in response to viable MmmSC. All the lymphocyte subsets as well as the monocyte/granulocyte subset exhibited extensive apoptosis after exposure to viable MmmSC. Our results demonstrated a potential role for MmmSC-secreted components as pathogenic factors able to induce programmed cell death in bovine blood leucocytes.
- Subjects
CONTAGIOUS bovine pleuropneumonia; CATTLE infections; CELL death; IMMUNE response; VETERINARY immunology; VETERINARY medicine
- Publication
Scandinavian Journal of Immunology, 2005, Vol 62, Issue 6, p528
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.2005.01690.x