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- Title
Extended storage of buffy coat platelet concentrates in plasma or a platelet additive solution.
- Authors
Slichter, Sherrill J.; Bolgiano, Doug; Corson, Jill; Jones, Mary Kay; Christoffel, Todd; Bailey, S. Lawrence; Pellham, Esther
- Abstract
Background Platelet ( PLT) concentrates ( PCs) prepared from whole blood in the United States are made using the PLT-rich plasma method. The PC s must be made within 8 hours of blood collection and stored for only 5 days. In Europe and Canada, PC s are made using the buffy coat ( BC) method from whole blood held overnight at 22°C and storage times may be up to 7 days. Our studies were designed to determine how long BC PLT s can be stored in plasma or Plasmalyte while meeting the FDA's poststorage viability criteria. Study Design and Methods Normal subjects donated whole blood that was stored at 22°C for 22 ± 2 hours before preparation of BC PLT s. PLT s were stored for 5 to 8 days in either plasma or Plasmalyte concentrations of 65 or 80%. Radiolabeled autologous stored versus fresh PLT recoveries and survivals were assessed as well as poststorage in vitro assays. Results BC PLT s stored in either plasma or 65% Plasmalyte met FDA poststorage PLT recovery criteria for 7 days but survivals for only 6 days, while storage in 80% Plasmalyte gave very poor results. Both stored PLT recoveries and survivals correlated with the same donor's fresh results, but the correlation was much stronger between recoveries than survivals. In vitro measures of extent of shape change, morphology score, and pH best predicted poststorage PLT recoveries, while annexin V binding best predicted PLT survivals. Conclusion BC PLT s stored in either plasma or 65% Plasmalyte meet FDA's poststorage viability criteria for 6 days.
- Subjects
BUFFY coat; BLOOD platelets; BLOOD plasma; DONOR blood supply; BLOOD collection; STORAGE; UNITED States. Food &; Drug Administration; GOVERNMENT policy
- Publication
Transfusion, 2014, Vol 54, Issue 9, p2283
- ISSN
0041-1132
- Publication type
Article
- DOI
10.1111/trf.12634