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- Title
Flow cytometric identification of 76 patients with biclonal disease among 5523 patients with chronic lymphocytic leukaemia (B- CLL) and its genetic characterization.
- Authors
Kern, Wolfgang; Bacher, Ulrike; Schnittger, Susanne; Dicker, Frank; Alpermann, Tamara; Haferlach, Torsten; Haferlach, Claudia
- Abstract
Multiparameter flow cytometry ( MFC) identifies rare cases of biclonal disease in chronic lymphocytic leukaemia ( CLL). By MFC, we identified 76 patients with biclonal disease in a cohort of 5523 CLL patients (1·4%). Fluorescence in situ hybridization and chromosome banding analysis revealed five and six cases, respectively, with two different cytogenetic aberrations due to clonal evolution. Two different B-cell receptor rearrangements and IGHV subtypes were more frequent in biclonal than in monoclonal CLL by MFC (37·1% vs. 2·7%; P < 0·001). Patients with biclonal CLL by MFC showed a trend to a shorter time to treatment than monoclonal CLL ( P = 0·080).
- Subjects
DIAGNOSTIC use of flow cytometry; CHRONIC lymphocytic leukemia diagnosis; CHRONIC lymphocytic leukemia; DIAGNOSTIC use of in-situ hybridization; CHROMOSOME banding; B cell receptors; PATIENTS
- Publication
British Journal of Haematology, 2014, Vol 164, Issue 4, p565
- ISSN
0007-1048
- Publication type
Article
- DOI
10.1111/bjh.12652