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- Title
The effect of exopolysaccharide-producing probiotic strains on gut oxidative damage in experimental colitis.
- Authors
Şengül, Neriman; Işık, Sevil; Aslım, Belma; Uçar, Gülberk; Demirbağ, Ali; Sengül, Neriman; Işık, Sevil; Aslım, Belma; Uçar, Gülberk; Demirbağ, Ali Eba
- Abstract
<bold>Background: </bold>Oxidative stress plays a role in disease initiation and progression in inflammatory bowel disease (IBD) and manipulation of this pathway may attenuate disease progress. In this study, the effect of exopolysaccharide (EPS)-producing probiotic bacteria on gut oxidative damage was evaluated in a rat model of experimental colitis.<bold>Methods: </bold>Colitis was induced by intracolonic administration of acetic acid. Rats were treated daily with two probiotic strains, L. delbrueckii subsp. bulgaricus B3 strain (EPS of 211 mg/l; high-EPS group) or L. delbrueckii subsp. bulgaricus A13 strain (EPS of 27 mg/l; low-EPS group), which were given directly into the stomach. Non-colitis-fed control and preventative groups were only treated with the high-EPS producing strain. Antioxidant enzyme activities (superoxide dismutase, catalase, total glutathione, reduced glutathione, glutathione disulfide) and lipid peroxidation were measured in colonic tissue samples after a treatment period of 7 days.<bold>Results: </bold>Significant oxidative damage was associated with a higher level of malondialdehyde (MDA) activity and reduced antioxidant enzyme activities in the colitis model group. All antioxidant enzyme activities were higher in both probiotic-treated groups compared with those of the colitis model group (P < 0.001). Lipid peroxidation was significantly ameliorated in both probiotic groups. The improvement of oxidative stress parameters was significantly more in the high-EPS group than in the low-EPS group (P < 0.001).<bold>Conclusions: </bold>EPS-producing probiotic bacteria significantly attenuate oxidative stress in experimental colitis. Increased EPS production gives rise to a better probiotic function. These results suggest that EPS molecules could revaluate probiotic strains and exert their beneficial effects on the host and this may have a therapeutic potential.
- Subjects
OXIDATIVE stress; CROHN'S disease; PROBIOTICS; MICROBIAL exopolysaccharides; EXPERIMENTAL design; LABORATORY rats; ENZYME activation; PREVENTION; COLITIS treatment; ACETIC acid; ANIMAL experimentation; ANIMALS; BACTERIAL antigens; BIOLOGICAL models; COLON (Anatomy); LACTOBACILLUS; LIPID peroxidation (Biology); POLYSACCHARIDES; RATS; MALONDIALDEHYDE
- Publication
Digestive Diseases & Sciences, 2011, Vol 56, Issue 3, p707
- ISSN
0163-2116
- Publication type
journal article
- DOI
10.1007/s10620-010-1362-7