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- Title
α-Tocopherol influences glycaemic control and <italic>miR-9-3</italic> DNA methylation in overweight and obese women under an energy-restricted diet: a randomized, double-blind, exploratory, controlled clinical trial.
- Authors
Luna, Rafaella Cristhine Pordeus; dos Santos Nunes, Mayara Karla; Monteiro, Mussara Gomes Cavalcante Alves; da Silva, Cássia Surama Oliveira; do Nascimento, Rayner Anderson Ferreira; Lima, Raquel Patrícia Ataíde; Pimenta, Flávia Cristina Fernandes; de Oliveira, Naila Francis Paulo; Persuhn, Darlene Camati; de Almeida, Aléssio Tony Cavalcanti; da Silva Diniz, Alcides; Pissetti, Cristina Wide; Vianna, Rodrigo Pinheiro Toledo; de Lima Ferreira, Flavia Emília Leite; Rodrigues Gonçalves, Maria da Conceição; de Carvalho Costa, Maria José
- Abstract
Background: Excess weight is a strong risk factor for the development of dysglycaemia. It has been suggested that changes in the metabolism microRNAs, small non-coding RNAs that regulate gene expression, could precede late glycaemic changes. Vitamin E in turn may exert important functions in methylation and gene expression processes. This study aimed to determine the effect of α-tocopherol on glycaemic variables and <italic>miR-9-1</italic> and <italic>miR-9-3</italic> promoter DNA methylation in overweight women. Methods: A randomized, double-blind, exploratory, placebo-controlled study was conducted in overweight and obese adult women (<italic>n</italic> = 44) who ingested synthetic vitamin E (all-rac-α-tocopherol), natural source vitamin E (RRR-rac-α-tocopherol) or placebo capsules and were followed up for a period of 8 weeks. Supplemented groups also received dietary guidance for an energy-restricted diet. An additional group that received no supplementation and did not follow an energy-restricted diet was also followed up. The intervention effect was evaluated by DNA methylation levels (quantitative real-time PCR assay) and anthropometric and biochemical variables (fasting plasma glucose, haemoglobin A1C, insulin, and vitamin E). Results: Increased methylation levels of the <italic>miR-9-3</italic> promoter region (<italic>P</italic> < 0.001) and reduced haemoglobin A1C (<italic>P</italic> < 0.05) were observed in the natural source vitamin E group after intervention. Increased fasting plasma glucose was observed in the synthetic vitamin E group, despite the significant reduction of anthropometric variables compared to the other groups. Conclusions: α-Tocopherol from natural sources increased methylation levels of the <italic>miR-9-3</italic> promoter region and reduced haemoglobin A1C in overweight women following an energy-restricted diet. These results provide novel information about the influence of vitamin E on DNA methylation. Trial registration: <ext-link>ClinicalTrials.gov</ext-link>, <ext-link>NCT02922491</ext-link>. Registered 4 October, 2016.
- Subjects
REDUCING diets; ANTHROPOMETRY; BLOOD sugar; DIET in disease; DIET therapy; FASTING; GLYCOSYLATED hemoglobin; INSULIN; POLYMERASE chain reaction; RESEARCH; VITAMIN E; RANDOMIZED controlled trials; BLIND experiment; DNA methylation; GLYCEMIC control
- Publication
Nutrition & Metabolism, 2018, Vol 15, Issue 1, pN.PAG
- ISSN
1743-7075
- Publication type
Article
- DOI
10.1186/s12986-018-0286-7