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- Title
Effects of Celangulin IV and V From Celastrus angulatus Maxim on Na<sup>+</sup>/K<sup>+</sup>-ATPase Activities of the Oriental Armyworm (Lepidoptera: Noctuidae).
- Authors
Dan Cheng; Mingxing Feng; Yufei Ji; Wenjun Wu; Zhaonong Hu
- Abstract
Na+/K+-ATPase (sodium pump) is an important target for the development of botanical pesticide as it is responsible for transforming chemical energy in ATP to osmotic work and maintaining electrochemical Na+and K+gradients across the cell membrane of most animal cells. Celangulin IV (C-IV) and V (C-V), which are isolated from the root bark of Celastrus angulatus, are the major active ingredients of this insecticidal plant. The activities of C-IV and C-V on Na+/K+-ATPase were investigated by ultramicro measuring method to evaluate the effects of C-IV and C-V on Na+/K+-ATPase activities of the brain from the fifth Mythimna separata larvae and to discuss the insecticidal mechanism of C-IV and C-V. Results indicate that inhibitory activities of Na+/K+-ATPase by C-IV and C-V possess an obvious concentration-dependent in vitro. Compared with C-IV, the inhibition of C-V on Na+/K+-ATPase was not striking. In vivo, at a concentration of 25 mg/liter, the inhibition ratio of C-IV on Na+/ K+-ATPase activity from the brain in narcosis and recovery period was more remarkable than that of C-V. Furthermore, the insects were fed with different mixture ratios of C-IV and C-V. The inhibition extent of Na+/K+-ATPase activity was corresponded with the dose of C-IV. However, C-V had no notable effects. This finding may mean that the mechanism of action of C-IV and C-V on Na+/K+-ATPase were different. Na+/K-ATPase may be an action target of C-IV and C-V.
- Subjects
SODIUM/POTASSIUM ATPase; MYTHIMNA separata; ULTRAMICROELECTRODES; SESQUITERPENES; ELECTROPHYSIOLOGY
- Publication
Journal of Insect Science, 2016, Vol 16, Issue 1, p1
- ISSN
1536-2442
- Publication type
Article
- DOI
10.1093/jisesa/iew051