We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Comparative investigation on the sizes and scavenger receptor binding of human native and modified lipoprotein particles with atomic force microscopy.
- Authors
Gan, Chaoye; Wang, Kun; Tang, Qisheng; Chen, Yong
- Abstract
Background: The size and receptor-binding abilities of plasma lipoproteins are closely related with their structure/functions. Presently, the sizes of native lipoproteins have been measured by various methods including atomic force microscopy (AFM) whereas the sizes of modified lipoproteins are poorly determined and the receptor-binding ability of lipoproteins is less detected and compared at the nanoscale. Methods: Here, AFM was utilized to detect/compare the size and scavenger receptor-binding properties of three native human lipoproteins including high-density lipoprotein, low-density lipoprotein (LDL), and very low-density lipoprotein, and two modified human lipoproteins including oxidized and acetylated LDL, as well as bovine serum albumin and their antibodies as negative and positive controls, respectively. Results: AFM detected that the sizes of these lipoproteins are close to the commonly known values and the previously-reported AFM-detected sizes and that native and modified LDL have different height/size. AFM also revealed that the CD36-binding abilities of the five lipoproteins are different from one another and from their SR-B1-binding abilities and that the anti-CD36/SR-B1 antibodies as positive controls have strong CD36/SR-B1-binding abilities. Conclusions: The data provide important information on lipoproteins for better understanding their structures/functions. Moreover, the data certify that besides size measurement AFM also can visualize receptor-lipoprotein binding at the nanoscale, as well as antigen–antibody (scavenger receptors and their antibodies) binding.
- Subjects
LIPOPROTEINS; SCAVENGER receptors (Biochemistry); ATOMIC force microscopy; ANTIGEN-antibody reactions; SERUM albumin
- Publication
Journal of Nanobiotechnology, 2018, Vol 16, p1
- ISSN
1477-3155
- Publication type
Article
- DOI
10.1186/s12951-018-0352-3