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- Title
MicroRNA - 34a induces transdifferentiation of glioma stem cells into vascular endothelial cells by targeting Notch pathway.
- Authors
Jin, Zaishun; Zhan, Tao; Tao, Jing; Xu, Biao; Zheng, Huizhe; Cheng, Yongxia; Yan, Bin; Wang, Hongwei; Lu, Guoqiang; Lin, Ying; Guo, Sufen
- Abstract
The function ofmicroRNA-34a(miR-34a) in transdifferentiation of glioma stem cells (GSCs) into vascular endothelial cells (VECs) was explored by focusing on Notch ligand Delta-like 1 (Dll1).MiR-34amimics was transfected into CD133 + glioma cell U251. The angiogenesis feature ofmiR-34atransfected U251 cells was investigated and the expressions of CD31, CD34, Vwf, Notch 1, and Dll1 were quantified. Length of branching vessel-like structures in themiR-34atransfected U251 cells was significantly higher than control cells. The VEC feature ofmiR-34aoverexpressed U251 cells was further confirmed by the expressions of CD31, CD34, and vWF. Transfection ofmiR-34adecreased the expression of Notch 1 and Dll1. Furthermore, themiR-34aoverexpression-enhanced tube formation of GSCs was suppressed when the decreased expression of Dll1 was restored. The current study highlighted the potential ofmiR-34aas an inducer in GSCs’ transdifferentiation into VECs by targeting Dll1. Up-regulation of miR-34a induced transdifferentiation of CD133+ U251 cells into VECs
- Subjects
MICRORNA; STEM cells; NOTCH genes
- Publication
Bioscience, Biotechnology & Biochemistry, 2017, Vol 81, Issue 10, p1899
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1080/09168451.2017.1364965