We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Analysis of genomic mutation and immunohistochemistry of platelet-derived growth factor receptors in canine vascular tumours.
- Authors
Abou Asa, S.; Mori, T.; Maruo, K.; Khater, A.; El‐sawak, A.; Abd el‐Aziz, E.; Yanai, T.; Sakai, H.
- Abstract
We examined whether mutation of the platelet-derived growth factor receptor protein tyrosine kinase ( PDGFR)-α and PDGFR-β genes contributes to their overexpression in canine vascular tumours. Genomic sequences of trans- or juxtamembrane regions of PDGFR-α and PDGFR-β were analysed with immunohistochemical staining and polymerase chain reaction-direct sequencing using DNA from paraffin-embedded neoplastic tissues of 27 hemangiosarcomas ( HSAs) and 20 hemangiomas ( HAs). Immunohistochemically, 75% of the HA cases were positive for PDGFR-α and almost most of the HA cases were negative for PDGFR-β. Of the HSA cases, 55.6% were negative for PDGFR-α and 63% were strongly positive for PDGFR-β. Among the HA cases, 1 missense mutation was detected in PDGFR-α exon 18 and 1 in PDGFR-β exon 17. Two HSA cases had missense mutations in exon 14 and 1 in exon 17 of PDGFR-β. Thus, genomic mutation of trans- or juxtamembrane regions of PDGFRs was not the main mechanism driving the activation of receptors in HSA and HA.
- Subjects
PLATELET-derived growth factor receptors; CANCER in dogs; GENETIC mutation; ANGIOSARCOMA; CANCER cell culture; DIAGNOSIS
- Publication
Veterinary & Comparative Oncology, 2015, Vol 13, Issue 3, p237
- ISSN
1476-5810
- Publication type
Article
- DOI
10.1111/vco.12035