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- Title
Isatis indigotica Induces Hepatocellular Cancer Cell Death via Caspase-Independent Apoptosis-Inducing Factor Translocation Apoptotic Pathway In Vitro and In Vivo.
- Authors
Chung, Ying-Cheng; Tang, Feng-Yao; Liao, Jiunn-Wang; Chung, Chia-Hua; Jong, Ting-Ting; Chen, Shih-Shiung; Tsai, Ching-Hsiu; Chiang, En-Pei
- Abstract
Isatis indigotica is a biennial herbaceous cruciferous medical herb with antipyretic, antiviral, anti-inflammatory, and anti-endotoxin activity. This study explored the chemotherapeutic potential of I indigotica on human hepatoma cells and investigated the mechanism by which metabolites from I indigotica inhibit hepatoma cell growth. Antitumor activity was discovered in dried I indigotica leaf chloroform extracts (CEDLI). In nude mice xenotransplanted with human hepatoma cells, CEDLI supplementation inhibited tumor growth by ~40% compared with nonsupplemented animals without affecting body weight/food intake. CEDLI induced sub-G1 cell cycle arrest and apoptosis in hepatoma cells. Furthermore, CEDLI activates p53 and Bax, reduces Bcl-2 expression, and causes mitochondrial stress and the release of apoptosis-inducing factor into the cytosol followed by its translocation into the nucleus, resulting in hepatoma cell apoptosis. This study provides novel in vivo evidence of I indigotica’s antitumor activity. The chemotherapeutic activity against human hepatoma tumorigenesis was because of a distinguished caspase-independent apoptotic pathway.
- Subjects
MEDICAL botany; ANALYSIS of variance; ANIMAL experimentation; ANTINEOPLASTIC agents; APOPTOSIS; BIOPHYSICS; COMPUTER software; ENZYME-linked immunosorbent assay; HEPATOCELLULAR carcinoma; HISTOLOGICAL techniques; IMMUNOHISTOCHEMISTRY; RESEARCH methodology; MEDICINAL plants; MICE; MICROSCOPY; RESEARCH funding; T-test (Statistics); TOXICITY testing; WESTERN immunoblotting; DATA analysis; PHARMACODYNAMICS
- Publication
Integrative Cancer Therapies, 2011, Vol 10, Issue 2, p201
- ISSN
1534-7354
- Publication type
Article
- DOI
10.1177/1534735410387420