We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Analysis of aqueous 2,4,6-trinitrotoluene (TNT) using a fluorescent displacement immunoassay.
- Authors
Goldman, Ellen R.; Anderson, George P.; Lebedev, Nikolai; Lingerfelt, Brian M.; Winter, Phan T.; Patterson, Charles H.; Mauro, J. Matthew
- Abstract
We report a rapid, simple, and sensitive assay that is potentially amenable to high throughput screening for analysis of 2,4,6-trinitrotoluene (TNT) present in aqueous solutions. The assay is based on the change in fluorescence emission intensity of a fluorescently labeled TNT analogue pre-bound to an anti-TNT antibody that occurs upon its competitive displacement by TNT. The assay can be performed in both cuvette- and 96-well plate-based formats. TNT at a level of 0.5 μg L–1 (0.5 ppb) was detected in phosphate buffered saline; detection improved to 0.05 μg L–1 (0.05 ppb) for TNT dissolved in artificial seawater.
- Subjects
TNT (Chemical); FLUORESCENCE; IMMUNOASSAY; ENVIRONMENTAL monitoring; SEAWATER; EXPLOSIVES
- Publication
Analytical & Bioanalytical Chemistry, 2003, Vol 375, Issue 4, p471
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-002-1713-0