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- Title
Chemical Deprenylation of N<sup>6</sup>‐Isopentenyladenosine (i<sup>6</sup>A) RNA.
- Authors
Cheng, Hou‐Ping; Yang, Xiao‐Hui; Lan, Ling; Xie, Li‐Jun; Chen, Chuan; Liu, Cuimei; Chu, Jinfang; Li, Zhi‐Yan; Liu, Li; Zhang, Tian‐Qi; Luo, Du‐Qiang; Cheng, Liang
- Abstract
N6‐isopentenyladenosine (i6A) is an RNA modification found in cytokinins, which regulate plant growth/differentiation, and a subset of tRNAs, where it improves the efficiency and accuracy of translation. The installation and removal of this modification is mediated by prenyltransferases and cytokinin oxidases, and a chemical approach to selective deprenylation of i6A has not been developed. We show that a selected group of oxoammonium cations function as artificial deprenylases to promote highly selective deprenylation of i6A in nucleosides, oligonucleotides, and live cells. Importantly, other epigenetic modifications, amino acid residues, and natural products were not affected. Moreover, a significant phenotype difference in the Arabidopsis thaliana shoot and root development was observed with incubation of the cation. These results establish these small organic molecules as direct chemical regulators/artificial deprenylases of i6A.
- Subjects
SMALL molecules; AMINO acid residues; RNA; RNA modification &; restriction; ROOT development; TRANSFER RNA; CYTOKININS
- Publication
Angewandte Chemie, 2020, Vol 132, Issue 26, p10732
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/ange.202003360