We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Hybrid Indicators for Fast and Sensitive Voltage Imaging.
- Authors
Xu, Yongxian; Peng, Luxin; Wang, Sicong; Wang, Anqi; Ma, Ruirui; Zhou, Ying; Yang, Jiahe; Sun, De‐en; Lin, Wei; Chen, Xing; Zou, Peng
- Abstract
Abstract: Membrane voltage is an important biophysical signal that underlies intercellular electrical communications. A fluorescent voltage indicator is presented that enables the investigation of electrical signaling at high spatial resolution. The method is built upon the site‐specific modification of microbial rhodopsin proteins with organic fluorophores, resulting in a hybrid indicator scaffold that is one of the most sensitive and fastest orange‐colored voltage indicators developed to date. We applied this technique to optically map electrical connectivity in cultured cells, which revealed gap junction‐mediated long‐range coupling that spanned over hundreds of micrometers.
- Subjects
FLUOROPHORES; RHODOPSIN; VISUAL pigments; PHYSICAL biochemistry; GAP junctions (Cell biology)
- Publication
Angewandte Chemie, 2018, Vol 130, Issue 15, p4013
- ISSN
0044-8249
- Publication type
Article
- DOI
10.1002/ange.201712614