We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Desulfurization of 2,4,6,8-tetraethyl dibenzothiophene by recombinant Mycobacterium sp. strain MR65.
- Authors
Watanabe, Kimiko; Noda, Ken-ichi; Konishi, Jin; Maruhashi, Kenji
- Abstract
Recombinant Mycobacterium sp. strain MR65 harboring dszABCD genes was used to desulfurize alkyl dibenzothiophenes (Cx-DBTs) in n-hexadecane. The specific desulfurization activity for 2,4,6,8-tetraethyl DBT (C8-DBT) by DszC enzyme was about twice that for 4,6-dipropyl DBT (C6-DBT). However, the degradation rate of 2,4,6,8-tetraethyl DBT in n-hexadecane by resting cells of strain MR65 was only about 40% of that of 4,6-dipropyl DBT. These results indicated that the desulfurization ability for Cx-DBTs by resting cells depends on carbon number substituted at positions 4 and 6 and that the rate-limiting step in the desulfurization reaction of highly alkylated Cx-DBTs is the transfer process from the oil phase into the cell.
- Subjects
MYCOBACTERIUM; DISULFIRAM; MYCOBACTERIA; GENES; STRAIN theory (Chemistry); ENZYMES
- Publication
Biotechnology Letters, 2003, Vol 25, Issue 17, p1451
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1025020003953