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- Title
Regulation of the xylanase gene, cgxA, from Chaetomium gracile by transcriptional factors, XlnR and AnRP.
- Authors
Rao, Uma; Marui, Junichro; Kato, Masashi; Kobayashi, Tetsuo; Tsukagoshi, Norihiro
- Abstract
The roles of XlnR and AnRP in regulating the expression of the xylanase gene, cgxA, from Chaetomium gracile were investigated using Aspergillus nidulans as an intermediate host. The XlnR consensus binding sequence –GGCTAA– in the promoter region was functional in vivo. The cgxA gene was induced when xylan was used as a carbon source but this inducibility was abolished when the XlnR binding sequence was mutated. Furthermore, the induction by xylan was increased when the AnRP binding sequence –TTGACAAAT– was mutated. Electrophoretic mobility shift assays using partially purified AnRP and an Aspergillus oryzae XlnR fusion protein, MalE-AoXlnR, provided evidence that the binding of the two proteins to their respective sites in the cgxA promoter region was mutually exclusive.
- Subjects
PROTEINS; BIOMOLECULES; GENE expression; GENETIC regulation; XYLANASES; GLYCOSIDASES
- Publication
Biotechnology Letters, 2002, Vol 24, Issue 13, p1089
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1016066617891