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- Title
Does Natural Clinoptilolite Induce Toxicity in Small Mammals?
- Authors
Beltcheva, Michaela; Tzvetanova, Yana; Todorova, Teodora; Tsvetanova, Liliya; Aleksieva, Iliana; Gerasimova, Tsvetelina; Chassovnikarova, Tsenka
- Abstract
The study aimed to assess the safety of using modified natural clinoptilolite as a food additive for mammals. Any potential adverse impacts of altered natural clinoptilolite on the regular physiological processes of animals were evaluated. Sixty male ICR laboratory mice, aged approximately 6–8 weeks, were randomly assigned to control and experimental groups. Both groups underwent a 45-day testing period, during which the experimental group was given rodent food mixed with 12.5% modified natural clinoptilolite from the Beli Plast deposit, Bulgaria. Sampling was carried out on days 0, 15, 30 and 45, with each time point providing samples for morpho-physiological and haematological studies, the oxidative stress on the base of malondialdehyde (MDA) and glutathione (GSH) measurements in liver and kidney so as the assessment of gene toxicity via the micronucleus test. All animals used in the experiment survived until the end of the study, gained weight and showed good activity and vital signs. The mice supplemented with clinoptilolite showed 21% higher body weight gain compared to the control group. Furthermore, there were no established signs of gene toxicity, pathological changes in the levels of oxidative stress and haematological parameters. These results suggest that using clinoptilolite as a food supplement does not lead to observable toxicities and even improves growth performance. Owing to its elevated sorption potential, this method is appropriate for purging the mammalian organism of toxins, with negligible adverse outcomes.
- Subjects
BULGARIA; CLINOPTILOLITE; FOOD additives; PATHOLOGICAL physiology; ANIMAL experimentation; LABORATORY mice; WEIGHT gain
- Publication
Acta Zoologica Bulgarica, 2024, Vol 76, Issue 2, p289
- ISSN
0324-0770
- Publication type
Article