We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
One-Pot Synthesis of Thermoresponsive Amyloidogenic Peptide-Polymer Conjugates via Thio-Bromo "Click" Reaction of RAFT Polymers.
- Authors
Kumar, Sonu; Deike, Stefanie; Binder, Wolfgang H.
- Abstract
A synthetic strategy to efficiently prepare main-chain peptide-polymer conjugates probing their aggregation in solution is described. An in situ tandem reaction based on aminolysis/thio-bromo "click" reaction is performed to tether an amyloidogenic peptide fragment amyloid-β17-20 (Leu-Val-Phe-Phe (LVFF)) to the ω-chain end of poly(diethylene glycol methyl ether acrylate) (PDEGA), prepared via reversible addition fragmentation chain transfer polymerization. Structural confirmation of the constructed conjugates PDEGA-LVFF (Mn,SEC = 5600, Đ = 1.21), (Mn,SEC = 7600, Đ = 1.16), and (Mn,SEC = 8900, Đ = 1.15) is successfully made by combined studies of ¹H NMR, size-exclusion chromatography, matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry, and electrospray ionization time-of-flight (ESI-TOF) mass spectrometry. The effect of the peptidic constituent on the thermoresponsive behavior of the polymer is examined by UV-vis spectros-copy, and the self-assembly behavior of the amphiphilic conjugate is further exploited, exhibiting micellar morphology in aqueous solution.
- Subjects
THERMORESPONSIVE polymers; CHEMICAL synthesis; CLICK chemistry; POLYMERIZATION; CHAIN transfer (Chemistry); ELECTROSPRAY ionization mass spectrometry
- Publication
Macromolecular Rapid Communications, 2018, Vol 39, Issue 2, p1
- ISSN
1022-1336
- Publication type
Article
- DOI
10.1002/marc.201700507