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- Title
Proteomic analysis of Streptomyces sp. ssr-198 grown on paddy straw.
- Authors
Singh, Surender; Tiwari, Rameshwar; Renuse, Santosh; Pranaw, Kumar; Nain, Lata
- Abstract
The filamentous bacteria Streptomyces spp. produces diverse extracellular enzymes and other secondary metabolites. Proteomic analysis of the secretome of holocellulolytic Streptomyces sp. ssr-198 was done by tandem mass spectrometry using an Orbitrap Velos hybrid mass spectrometer. A wide range of hydrolytic enzymes, including glycoside hydrolases (17), proteases (17), polysaccharide lyases (3), esterases (2), and hypothetical proteins (14) were detected in the secretome analyzed. Overall, the secretome composition constituted of 12.50% cellulases, 17.50% hemicellulases, 21.25% proteases, 17.50% hypothetical proteins, and 31.25% other proteins. Comprehensive analysis of secretome will be useful in gaining better understanding of the unique role of hydrolytic enzymes in lignocellulose hydrolysis and helps in determining the industrial applications of these potent enzymes.
- Subjects
STREPTOMYCES; VOLVARIELLA volvacea; EXTRACELLULAR enzymes; HYDROLASE genetics; HYDROLASES regulation; PHYSIOLOGY
- Publication
Journal of Basic Microbiology, 2015, Vol 55, Issue 6, p790
- ISSN
0233-111X
- Publication type
Article
- DOI
10.1002/jobm.201400639