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- Title
Trichoderma longibrachiatum and thermothelomyces thermophilus co-culture: improvement the saccharification profile of different sugarcane bagasse varieties.
- Authors
Contato, Alex Graça; Nogueira, Karoline Maria Vieira; Buckeridge, Marcos Silveira; Silva, Roberto Nascimento; Polizeli, Maria de Lourdes Teixeira de Moraes
- Abstract
Objectives: The aim of the present work was to perform the co-culture between Trichoderma longibrachiatum LMBC 172, a mesophilic fungus, with Thermothelomyces thermophilus LMBC 162, a thermophilic fungus, by submerged fermentation in a bioreactor. Results: There was an increase in protein production, reaching the value of 35.60 ± 3.76 µg/ml at 72 h. An increase in the amount of proteins of 27.5% in relation to the isolated cultivation of T. longibrachiatum and 19.7% in comparison when T. thermophilus was isolated and cultivated. After that, the saccharification profile of three varieties of sugarcane (sugarcane in natura, culms of sugarcane SP80-3280, and culms of Energy cane) submitted in two pretreatments (autohydrolysis and chemical) was performed. The (e) chemical pretreatment was the better in generating of fermentable sugars from sugarcane bagasse and culms of Energy cane, while with the autohydrolysis pretreatment was obtained the better values to culms of SP80-3280 sugarcane. The sugars found were glucose, xylose, arabinose, and cellobiose. Conclusion: These results suggest that the co-culture between these microorganisms has the potential to produce an enzymatic cocktail with high performance in the hydrolysis of materials from the sugar-alcohol industry.
- Subjects
STREPTOCOCCUS thermophilus; SUGARCANE; BAGASSE; THERMUS thermophilus; TRICHODERMA; THERMOPHILIC fungi; ARABINOSE
- Publication
Biotechnology Letters, 2023, Vol 45, Issue 9, p1093
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-023-03395-7