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- Title
ESTRESSE HÍDRICO IN VITRO EM ACESSOS DE JENIPAPEIRO.
- Authors
Nogueira de Araújo, Anna Beatriz; de Souza Nascimento, Augusto Vinicius; Almeida da Graça, Genilza; de Melo Massaranduba, Wendel; Rodrigues Santos, Marielly; Cruz da Silva, Ana Veruska; da Silva Ledo, Ana
- Abstract
Genipa americana L., known as genipap, is an endemic species of the Brazilian Northeast, particularly abundant in the state of Sergipe, and has attracted interest due to its diversity of uses. This study aimed to investigate the response of two distinct genipap accessions from the Cerrados region (Núcleo Bandeirante and Park Way) to hydric stress conditions in a controlled in vitro environment. For this purpose, five concentrations of mannitol (0, 100, 200, 300 and 400 mM) were employed as osmotic agent. The results obtained revealed a significant similarity in survival rates among the evaluated genotypes. While the control group, without the addition of mannitol, recorded the highest survival rate at 95%, the higher concentrations (300 and 400 mM) showed a decrease in this index, reaching only 70% for 300 mM and 65% for 400 mM. A distinctive aspect emerged regarding the length of adventitious shoots, with the NB accession associated with greater length compared to the PK accession. Regarding to rooting, survival, callus formation and oxidation, no significant differences were evidenced between the accessions. The increase in mannitol concentrations led to a reduction in leaf count. At the concentration of 400 mM, the lowest number of leaves was recorded, suggesting that such concentrations induce stress levels in the explant. Concerning the development of adventitious shoots, an increment was observed from 0 mM up to 200 mM of mannitol. However, from 300 mM, there was a negative linear regression for the number of shoots. It was observed that the increase in mannitol concentrations resulted in an inhibition of adventitious shoot growth. The use of mannitol as an osmotic agent for in vitro culture proved to be a promising strategy to induce stress responses. These results contribute to a broader understanding of the physiology and stress response of genipap, offering valuable insights for potential future applications in a breeding programs.
- Subjects
STRESS concentration; ENDEMIC species; MANNITOL; SURVIVAL rate; CALLUS
- Publication
Revista Foco (Interdisciplinary Studies Journal), 2023, Vol 16, Issue 9, p1
- ISSN
1981-223X
- Publication type
Article
- DOI
10.54751/revistafoco.v16n9-144