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- Title
Long Non-Coding RNA Modulation of VEGF-A during Hypoxia.
- Authors
Nieminen, Tiina; Scott, Tristan A.; Feng-Mao Lin; Zhen Chen; Yla-Herttuala, Seppo; Morris, Kevin V.
- Abstract
The role and function of long non-coding RNAs (lncRNAs) in modulating gene expression is becoming apparent. Vascular endothelial growth factor A (VEGF-A) is a key regulator of blood vessel formation and maintenance making it a promising therapeutic target for activation in ischemic diseases. In this study, we uncover a functional role for two antisense VEGF-A lncRNAs, RP1-261G23.7 and EST AV731492, in transcriptional regulation of VEGF-A during hypoxia. We find here that both lncRNAs are polyadenylated, concordantly upregulated with VEGF-A, localize to the VEGF-A promoter and upstream elements in a hypoxia dependent manner either as a single-stranded RNA or DNA bound RNA, and are associated with enhancer marks H3K27ac and H3K9ac. Collectively, these data suggest that VEGF-A antisense lncRNAs, RP1-261G23.7 and EST AV731492, function as VEGF-A promoter enhancer-like elements, possibly by acting as a local scaffolding for proteins and also small RNAs to tether.
- Subjects
NON-coding RNA; VASCULAR endothelial growth factors; ENDOTHELIAL growth factors; POLYNUCLEOTIDE adenylyltransferase; BLOOD vessels
- Publication
Non-Coding RNA, 2018, Vol 4, Issue 4, p1
- ISSN
2311-553X
- Publication type
Article
- DOI
10.3390/ncrna4040034