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- Title
Identification of functional domains of phosphoproteins of two morbilliviruses using chimeric proteins.
- Authors
M. S. Shaila
- Abstract
Abstract The paramyxovirus P protein is an essential component of the transcriptase and replicase complex along with L protein. In this article, we have examined the functional roles of different domains of P proteins of two closely related morbilliviruses, Rinderpest virus (RPV) and Peste des petits ruminants virus (PPRV). The PPRV P protein physically interacts with RPV L as well as RPV N protein when expressed in transfected cells, as shown by co-immunoprecipitation. The heterologous L–P complex is biologically active when tested in a RPV minigenome replication/transcription system, only when used with PPRV N protein but not with RPV N protein. Employing chimeric PPRV/RPV cDNAs having different coding regions of P protein in the minigenome replication/transcription system, we identified a region between 290 and 346 aa in RPV P protein necessary for transcription of the minigenome.
- Subjects
PARAMYXOVIRUSES; PROTEINS; RINDERPEST virus; PESTE des petits ruminants
- Publication
Virus Genes, 2008, Vol 37, Issue 1, p1
- ISSN
0920-8569
- Publication type
Article