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- Title
农杆菌介导的小孢拟盘多毛孢遗传转化及突变体筛选.
- Authors
邓蕾; 冯丹丹; 汪祖鹏; 潘慧; 钟彩虹; 李黎
- Abstract
The study of Agrobacterium tumefaciens EHA105 mediated transformation of Pestalotiopsis microspora KFRD-2 was carried out by using recombinant vector pCAMBgfp containing hygromycin resistance gene and GFP gene. Based on hygromycin and GFP fluorescence resistance, the transformants were screened. Through the analysis of colony morphology, mycelium growth rate, sporulation, fluorescence stability and pathogenicity verification, more than 100 positive transformants were screened out. The average transformation efficiency was up to 200 transformants per 106 conidia. Most transformants were no significant differences in morphological characteristics and pathogenicity as compared with the wild-type strains. Fourteen positive mutants with significant differences in mycelial morphological characteristics, sporulation or pathogenicity were obtained, which could be used to explore the virulence genes and the pathogenic mechanism of P. microspora.
- Subjects
GREEN fluorescent protein; MICROSPORIDIA; AGROBACTERIUM; CONIDIA; FLUORESCENCE; AGROBACTERIUM tumefaciens; PLANT genetic transformation
- Publication
Mycosystema, 2021, Vol 40, Issue 9, p2355
- ISSN
1672-6472
- Publication type
Article
- DOI
10.13346/j.mycosystema.210120