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- Title
长链非编码RNA 浆细胞瘤变异性易位基因1 参与盆腔器官脱垂的机制.
- Authors
胡新明; 乔艳华; 王肖帆; 李林玉; 赵 冰
- Abstract
BACKGROUND: Previous studies have shown that the expression of long non-coding RNA plasmacytoma variant translocation 1 (LncRNA PVT1) was significantly reduced in the uterine cardinal ligament of patients with pelvic organ prolapse through high-throughput sequencing. Transforming growth factor β1 signaling pathway is also one of the related pathways. OBJECTIVE: To investigate the effect of LncRNA PVT1 on pelvic floor ligament fibroblasts and its possible role in the pathogenesis of pelvic organ prolapse. METHODS: Tissue samples of the pelvic floor ligament were extracted from rats and fibroblasts were isolated for primary culture. LncRNA PVT1 interference and overexpression vectors were constructed by lentivirus and added into fibroblasts. Morphology, proliferation, migration and apoptosis of fibroblasts were detected by immunofluorescence, MTT, cell scratch assay and flow cytometry test respectively. The mRNA and protein expressions of transforming growth factor-β1, Smad2/3 and c-Myc were detected by RT-PCR and western blot respectively. RESULTS AND CONCLUSION: Overexpression of LncRNA PVT1 could significantly promote the proliferation of fibroblasts, improve the migration ability of cells, and inhibit the apoptosis of fibroblasts. The results of LncRNA PVT1 interference were opposite. In addition, the mRNA and protein relative expressions of c-Myc, transforming growth factor-β1 and Smad2/3 were significantly increased when LncRNA PVT1 gene was overexpressed, while the expressions of c-Myc and transforming growth factor-β1 were significantly inhibited when LncRNA PVT1 gene was interfered. To conclude, LncRNA PVT1 can affect the morphology and biological function of fibroblasts, which may be related to the transforming growth factor-β1/Smad2/3/c-Myc pathway. This study provides new ideas for the clinical diagnosis and treatment of pelvic organ prolapse.
- Subjects
PELVIC organ prolapse; PELVIC floor; GENE expression; NUCLEOTIDE sequencing; PROTEIN expression; LINCRNA; TRANSFORMING growth factors; CELL migration
- Publication
Chinese Journal of Tissue Engineering Research / Zhongguo Zuzhi Gongcheng Yanjiu, 2023, Vol 27, Issue 5, p669
- ISSN
2095-4344
- Publication type
Article
- DOI
10.12307/2023.092