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- Title
Co-ordinated Regulations of mRNA Synthesis and Decay during Cold Acclimation in Arabidopsis Cells.
- Authors
Toshihiro Arae; Shiori Isai; Akira Sakai; Katsuhiko Mineta; Masami Yokota Hirai; Yuya Suzuki; Shigehiko Kanaya; Junji Yamaguchi; Satoshi Naito; Yukako Chiba
- Abstract
Plants possess a cold acclimation system to acquire freezing tolerance through pre-exposure to non-freezing low temperatures. The transcriptional cascade of C-repeat-binding factors (CBFs)/dehydration response element-binding factors (DREBs) is considered a major transcriptional regulatory pathway during cold acclimation. However, little is known regarding the functional significance of mRNA stability regulation in the response of gene expression to cold stress. The actual level of individual mRNAs is determined by a balance between mRNA synthesis and degradation. Therefore, it is important to assess the regulatory steps to increase our understanding of gene regulation. Here, we analyzed temporal changes in mRNA amounts and half-lives in response to cold stress in Arabidopsis cell cultures based on genomewide analysis. In this mRNA decay array method, mRNA halflife measurements and microarray analyses were combined. In addition, temporal changes in the integrated value of transcription rates were estimated from the above two parameters using a mathematical approach. Our results showed that several cold-responsive genes, including Cold-regulated 15a, were relatively destabilized, whereas the mRNA amounts were increased during cold treatment by accelerating the transcription rate to overcome the destabilization. Considering the kinetics of mRNA synthesis and degradation, this apparently contradictory result supports that mRNA destabilization is advantageous for the swift increase in CBF-responsive genes in response to cold stress.
- Subjects
ARABIDOPSIS thaliana; MESSENGER RNA; GENETIC regulation; PHYSIOLOGICAL effects of cold temperatures; CYSTATHIONINE gamma-lyase
- Publication
Plant & Cell Physiology, 2017, Vol 58, Issue 6, p1090
- ISSN
0032-0781
- Publication type
Article
- DOI
10.1093/pcp/pcx059