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- Title
Zuverlässigkeit der Blutgruppenbestimmung bei Neugeborenen.
- Authors
Bahr, E. A.; Kretschmer, V.
- Abstract
2209 blood group determinations, 1121 times using cord blood and 1088. times using venous blood, were performed with two different methods: A "short blood group determination" as slide test with the pellet of unwashed erythrocytes and a panel of antisera including ant/-A, -B, -AB, and Rh-serum control without reverse grouping using a Rhesus box for .Rhesus testing. An "extended blood group determination" was performed with erythrocytes washed one time and re- suspended in normal saline solution with two different panels of antisera. The determination of the ABO- and Rh-antigens was done on Bioplates®. Reverse grouping was performed in a three step procedure: saline test (22°C), albumin test (37°C) and indirect antiglobulin test. In 11 out of 1590 cases (0.69%) a false blood group was determined with the "short A BO typing procedure". Non-specific agglutination (6 cases) and weak agglutination because of an unsufficiently developed antigen A (5 cases) appeared as main reasons for wrong ABO-typing. The frequency of mistyping with this method was higher with cord blood than with venous blood (0.97% versus 0.18%; p < 0.05). Wrong typing of the Rh-factor was observed in the "short procedure" in 12 out of 1590 cases (0.75%). Because of weak reaction of the D-antigen more often Rh-negative was determined instead of Rh-positive (8 cases) than vice versa (4 cases). Non-specific agglutination misled false positive Rh-typing in 4 cases. Mistyping was more frequent with venous blood than with cord blood (1.06% versus 0.58%; p> 0.10). Reverse grouping was valuable for the ABO-determination. In 29.3% of the blood groups A, B and 0 the compatible alloantibodies were detected with the saline test. With the albumin test the frequency increased to 32.6% and with the antiglobulin test to 57.6%. Incompatible AB-antibodies were found in only 0 88% of the blood groups A and B with the saline test in 1.1% with the albumin test but in 11.1% with the indirect antiglobulin test. Blood group typing ofnewborns should be performed with the "extended blood group determination" which includes reverse grouping with the saline test. Reverse grouping with the indirect antiglobulin test is recommended for the special immunohematological analysis of ABO incompatibility.
- Publication
Journal of Laboratory Medicine / Laboratoriums Medizin, 1986, Vol 10, Issue 5, p134
- ISSN
0342-3026
- Publication type
Article