We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
HPLC-PDA を用いた北海道産ジビエ中プラジクアンテルの 残留分析法の開発.
- Authors
平田祥太郎; 昌山敦; 仲谷正; 高取聡; 星英之
- Abstract
An analytical method that uses HPLC was developed to analyze residual praziquantel in game meats hunted in Hokkaido. Samples were initially extracted with acetonitrile/methanol and McIlvaine buffer containing EDTA. The impurities were removed from the extract by n-hexane partition and solid-phase column. After removing the solvent, the extract was resolved in a 40% acetonitrile solution. Praziquantel in the purified solution was quantitatively determined using HPLC with a photodiode array detector (PDA). An interfering peak was not observed in chromatograms of game meat extracts, indicating the high selectivity of this method. Method validation was performed by recovery tests at the maximum residue limit concentration to evaluate the trueness, repeatability (RSDr), and with-in laboratory reproducibility (RSDwr). The validation results exhibited sufficient recovery (range, 84-91%) and precision (RSDr ≤ 6% and RSDwr ≤ 12%). These values fulfilled the criteria for the validation guidelines for residual pesticide analysis in Japan. The limit of quantification of this method was 0.03 μg/g in game meats. The time required for the preparation of eight test sample solutions was less than five hours. This method can help inspect residual praziquantel in game meats hunted in Hokkaido.
- Subjects
JAPAN; ACETONITRILE; PRAZIQUANTEL; CHROMATOGRAMS; HIGH performance liquid chromatography; PESTICIDES; ETHYLENEDIAMINETETRAACETIC acid; MEAT analysis; ORGANOPHOSPHORUS pesticides
- Publication
Japanese Journal of Food Chemistry & Safety, 2023, Vol 30, Issue 3, p121
- ISSN
1341-2094
- Publication type
Article