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- Title
PARP-1 对高糖诱导的心肌细胞增殖的影响及机制研究.
- Authors
李青; 刘昕; 范博渊; 倪雅娟; 刘平
- Abstract
Objective: The study the effect and mechanismof PARP-1 on the proliferation of H9C2 cells induced by high glucose. Methods:H9C2 cells were treated with high glucose, and the levels of PARP-1 mRNA and protein in the cells were detected by qRT-PCR and Western blot. H9C2 cells were transfected PARP-1 siRNA and siRNA control, qRT-PCR and Western blot were used to detect PARP-1 mRNA and protein levels in the cells. H9C2 cells were transfected with PARP-1 siRNA and high glucose. CCK-8 was used to detect the cell proliferation, thiobarbituric acid method was used to detect the level of malondialdehyde. The level of SOD was detected by xanthine oxidase method. The levels of PCNA, p38MAPK and p-p38MAPK protein were detected by Western blot. Results: The level of PARP-1 mRNA and protein in H9C2 cells after high glucose was significantly higher than that of normal cultured H9C2 cells (P<0.05). PARP-1 siRNA can obviously reduce the level of PARP-1 mRNA and protein in H9C2 cells. The survival rate of H9C2 cells decreased significantly after high glucose treatment. The level of MDA in the cells was elevated, the level of SOD in the cells was reduced, the level of PCNA in the cells decreased and the level of p38MAPK phosphorylation increased, compared with the normal H9C2 cells, the difference was statistically significant (P<0.05). Down-regulation of PARP-1 H9C2 cells with high glucose, the survival rate of cells increased, the level of MDA in the cells was reduced, the level of SOD also increased in the cells, the level of PCNA in the cells was elevated, the level of p38MAPK phosphorylation in the cells was reduced, compared with pure high sugar cells, the difference was statistically significant (P<0.05). Conclusion: The expression of PARP-1 was up-regulated in cardiomyocytes induced by high glucose, which increase the cell lipid oxidation stress to inhibit the proliferation of cardiomyocytes by activating p38MAPK signal pathway.
- Subjects
XANTHINE oxidase; CELL proliferation; WESTERN immunoblotting; SMALL interfering RNA; GLUCOSE; SUGAR
- Publication
Progress in Modern Biomedicine, 2019, Vol 19, Issue 4, p642
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2019.04.008