We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Pedilanthus tithymaloides, an Indian Folklore, Inhibits HSV Infection.
- Authors
Ojha, Durbadal; Goswami, Debayan; Chattopadhyay, Debprasad
- Abstract
Pedilanthus tithymaloides (PT), a widely used ethnomedicinal plant, has been employed to treat a number of skin conditions. To validate its ethnomedicinal potential, we have evaluated the in vitro and in vivo antiviral activity of a methanolic extract of PT leaves and its isolated compound luteolin against Herpes Simplex Virus type-1. The MTT and plaque assay showed that both the extract and luteolin had potent antiviral activity against wild-type and clinical isolates of HSV-1 (EC50, 43.6- 52.6 and 19.2-27.5 pg/ml, respectively). The inhibitory effect was significant when the extract or compound were added at 2 h prior to, during or 4 h post-infection, indicating that the mode of action of luteolin is not only the viral entry but also in the post-entry events or host cell modulation. Earlier studies have reported that NF-κB has role in activation for effective replication of HSV and its suppression to inhibit HSV infection. We have demonstrated that the extract or luteolin suppress the NF-κB activation dining HSV-1 infection. The in vivo studies revealed that the extract or luteolin at its nontoxic concentration reduced virus yield in the brain of HSV-1 infected mice, compared to skin tissues. Furthermore, both extract and luteolin significantly reduced TNF-α, IL-lβ, IL-6, and IFN-γ expression in HSV-1 infected drug-treated Trigeminal Ganglia, related to TLR3 signaling. Interestingly, following HSV infection luteolin treatment decreased the TLR3 mRNA expression but inhibited upregulation of TRIF and NF-κB expression. These results collectively suggested that the extract or luteolin treatment altered HSV induced inflammation via a TLR3 signaling pathway
- Subjects
MEDICINAL plants; HERPES simplex transmission; SKIN disease treatment
- Publication
Journal of Ravishankar University, Part-B Science, 2016, Vol 29, p95
- ISSN
0970-5910
- Publication type
Abstract